Wheat Germ Topoisomerase I
Wheat germ topoisomerase I is prepared from wheat germ and is capable of relaxing supercoiled DNA.
1 U of topo I will relax 0.5 µg of supercoiled pBR322 DNA in 1X Assay buffer in a total reaction volume of 30 µl in 30 minutes at 37°C.
Store at -80ºC.
All enzyme is supplied with Dilution Buffer and 2X concentrated Assay Buffer which are also available separately.
Please enquire for larger quantities/bulk orders.
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