Wheat Germ Topoisomerase I

Wheat germ topoisomerase I is prepared from wheat germ and is capable of relaxing supercoiled DNA.

1 U of topo I will relax 0.5 µg of supercoiled pBR322 DNA in 1X Assay buffer in a total reaction volume of 30 µl in 30 minutes at 37°C. 

Store at -80ºC.

All enzyme is supplied with Dilution Buffer and 2X concentrated Assay Buffer which are also available separately.

Please enquire for larger quantities/bulk orders.