Human Topoisomerase I

Human DNA Topoisomerase I (Topo I) is a type IB topoisomerase which can relax supercoiled DNA. It is prepared by overexpressing the enzyme in baculovirus-infected insect cells (Spodoptera frugiperda) and purifying it by methods adapted from Stewart et al., 1996¹. The enzyme is supplied in Dilution Buffer.

1 U of topo I will relax 0.5 µg supercoiled pBR322 DNA in 1X Assay buffer in a total reaction volume of 30 µl in 30 minutes at 37°C.

This enzyme is supplied with 10X concentrated Assay Buffer and Dilution buffer which are also available separately.

Store at -80°C. 

Please enquire for larger quantities/bulk orders.

See technical documents below for more detailed information and lot specific activities.

 

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References

1. Stewart, L., Ireton, G.C., Parker, L.H., Madden, K.R. and Champoux, J.J. (1996). Biochemical and biophysical analyses of recombinant forms of human topoisomerase I. J. Biol. Chem. 271: 7593-7601