M. tuberculosis Topoisomerase I Relaxation Kit

These kits contain everything required for relaxation reactions E. coli Topoisomerase IV enzyme, the supercoiled pBR322 DNA substrate, the Assay and Dilution buffers as well as 10X GSTEB - our new stop and loading buffer.

Mycobacterium tuberculosis DNA Topoisomerase I (Topo I) is a bacterial type IA topoisomerase which can relax supercoiled DNA. It is a potential target for antibacterial drugs and can be used for screening potential novel antibacterial compounds. It is prepared by overexpressing the protein in E. coli and then purified by methods developed in-house. The enzyme is supplied in Dilution Buffer.

1 U of topo I will relax 0.5 µg supercoiled pBR322 DNA in 1X Assay buffer in a total reaction volume of 30 µl in 30 minutes at 37°C.

Store at -80°C.

Please enquire for larger quantities/bulk orders.

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Cat no.	Product	Price	Quantity
MTTR01	M. tuberculosis Relaxation Kit 1 100 U of M. tuberculosis Topo I supplied with 5X Assay Buffer, Dilution Buffer, 50 µg negatively supercoiled pBR322 plasmid, and 10X GSTEB	£482.00
MTTR02	M. tuberculosis Relaxation Kit 2 500 U of M. tuberculosis Topo I supplied with 5X Assay Buffer, Dilution Buffer, 250 µg negatively supercoiled pBR322 plasmid, and 10X GSTEB	£1,730.00