Human Topoisomerase II Beta Decatenation Assay Kits

These kits contain all the components necessary to perform decatenation reactions. Including the enzyme, the catenated BisCat DNA or kDNA substrate, a decatenated kDNA marker (for kDNA kits), the Assay and Dilution buffers as well as 10X GSTEB - our new stop and loading buffer.

Our NEW BisCat DNA decatenation substrate consists of two supercoiled, singly-linked minicircles of 2.3 and 2.6 kb. Type II topoisomerases can decatenate the substrate to produce two supercoiled minicircle products.

Human DNA Topoisomerase II beta (Topo IIβ) is a type II topoisomerase that can decatenate and relax supercoiled DNA. It is prepared by overexpressing in baculovirus-infected insect cells (Spodoptera frugiperda) and purifying it by methods developed in-house.

The enzyme is supplied in Dilution Buffer.

1 U of topo IIβ will decatenate 200 ng of BisCat or kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes.

Please enquire for larger quantities/bulk orders.

See technical documents below for more detailed information and lot specific activities.

Cat no.	Product	Price
HTBD201	Human Topoisomerase II Beta BisCat Decatenation Kit 1 Contains 100 U of human topo II beta, 20 µg BisCat DNA, ATP, Assay Buffer, Dilution Buffer, and 10X GSTEB	£467.00
HTKB201	Human Topoisomerase II Beta kDNA Decatenation Kit 1 Contains 100 U of human topo II beta, 20 µg kDNA, ATP, Assay Buffer, Dilution Buffer, decatenated kDNA marker and 10X GSTEB	£437.00
HTBD202	Human Topoisomerase II Beta BisCat Decatenation Kit 2 Contains 500 U of human topo II beta, 100 µg BisCat DNA, ATP, Assay Buffer, Dilution Buffer, and 10X GSTEB	£1,485.00
HTKB202	Human Topoisomerase II Beta kDNA Decatenation Kit 2 Contains 500 U of human topo II beta, 100 µg kDNA, ATP, Assay Buffer, Dilution Buffer, decatenated kDNA marker and 10X GSTEB	£1,343.00
HTBD203	Human Topoisomerase II Beta BisCat Decatenation Kit 3 Contains 1000 U of human topo II alpha, 200 µg BisCat DNA, ATP, Assay Buffer, Dilution Buffer, and 10X GSTEB	£2,194.00
HTKB203	Human Topoisomerase II Beta kDNA Decatenation Kit 3 Contains 1000 U of human topo II beta, 200 µg kDNA, ATP, Assay Buffer, Dilution Buffer, decatenated kDNA marker and 10X GSTEB	£2,042.00
HTDB204	Human Topoisomerase II Beta BisCat Decatenation Kit 4 Contains 2000 U of human topo II beta, 400 µg BisCat DNA, ATP, Assay Buffer, Dilution Buffer, and 10X GSTEB	£3,228.00
HTKB204	Human Topoisomerase II Beta kDNA Decatenation Kit 4 Contains 2000 U of human topo II beta, 400 µg kDNA, ATP, Assay Buffer, Dilution Buffer, decatenated kDNA marker and 10X GSTEB	£3,003.00

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References

Waraich NF, Jain S, Colloms SD, Stark WM, Burton NP, Maxwell A. A novel decatenation assay for DNA topoisomerases using a singly-linked catenated substrate. Biotechniques. 2020 Nov;69(5):356-362.