Human Topoisomerase II Beta Decatenation Assay Kits
These kits contain all the components necessary to perform decatenation reactions. Including the enzyme, the catenated kDNA substrate, a decatenated kDNA marker, the Assay and Dilution buffers as well as 10X GSTEB - our new stop and loading buffer.
Human DNA Topoisomerase II beta (Topo IIb) is a type II topoisomerase that can decatenate and relax supercoiled DNA. It is prepared by overexpressing in baculovirus-infected insect cells (Spodoptera frugiperda) and purifying it by methods developed in-house.
The enzyme is supplied in Dilution Buffer.
1 U of topo IV will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes.
Please enquire for larger quantities/bulk orders.
See technical documents below for more detailed information and lot specific activities.
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