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Zika virus NS2B-NS3 Protease Assay Kits

Polyprotein processing protease enzymes from Flaviviridae group are considered important drug targets. Zika virus NS2B-NS3 protease recognises a specific amino acid sequence valine-lysine-lysine-arginine (VKKR) and processes the polyprotein into individual functional proteins or peptides. However, this protease can also recognise and digest valine-lysine-arginine-arginine (VKRR). Using this kit the Zika virus NS3-NS2B protease activity is tested against a Benzoyl−norleucine−lysine−arginine−arginine−7-amino-4-methylcoumarin (Bz-Nle-KRR-AMC) fluorescent substrate. Digestion of the substrate Bz-Nle-KRR-AMC by the protease will release fluorescent AMC and the increase in fluorescence can be read by measuring the 440-460 nm emission after excitation at 340-360 nm. 

These assays are performed in 96 well microtitre plates and contain the NS3 protease, the substrate Bz-Nle-KRR-AMC, assay and dilution buffers and microtitre plates.

We have a range of products that will help your drug discovery efforts, whether you are looking for novel anti-infectives, or new anti-cancer compounds, we have a range of target enzymes, available with and without their substrates, in easy to use kits. We also supply different topological isoforms of our DNA substrates.

Don't see your required enzyme? Contact us to see if we can make it for you or visit our Custom Proteins page! 

Not doing drug discovery? Our enzymes and DNA substrates are of extremely high purity and quality, perfect for reliable results!

All of our enzymes can be supplied in large or bulk quantities or at higher concentrations. Please contact us for a quote!

Likewise, if you require enzymes with particular specifications (e.g. low endotoxin, DNase or RNase free, low bioburden etc.), please contact us.


You can contact us on +44(0) 1603 673590 or +44(0) 1603 673591, at [email protected] or by visiting our contact us page to discuss your requirements.

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