Yeast Topoisomerase II

• Yeast DNA Topoisomerase II (Topo II) - a type II topoisomerase essential for DNA replication, transcription and chromosome segregation
• Performs both relaxation and decatenation of supercoiled DNA
• Produced by overexpression in Saccharomyces cerevisiae and purified using proprietary in-house methods
• Supplied as a high-purity, activity-validated enzyme in dilution buffer
• 1 Unit relaxes 0.5 µg of supercoiled pBR322 DNA in 30 minutes at 37°C
• Includes purified enzyme, 5X Assay Buffer and Dilution Buffer (available separately)
• Relaxation kits include enzyme, DNA substrate, buffers, and 10X GSTEB stop and loading buffer
• Ideal for relaxation and decatenation assays, mechanistic studies, and inhibitor screening
• Suitable for comparative studies with DNA gyrase and other topoisomerases
• High reproducibility and lot-to-lot consistency, supported by detailed QC data
• Long-term stability when stored at -80°C
• Bulk and custom quantities available for academic or pharmaceutical research

Yeast DNA Topoisomerase II (also referred to as topoisomerase II, topoisomerase 2, or DNA topoisomerase 2) is a type II topoisomerase that plays a vital role in maintaining DNA topology. Topo II regulates DNA supercoiling and enables decatenation, processes that are essential for DNA replication, transcription, and chromosome segregation. Due to these critical functions, topoisomerase II is widely studied as a model enzyme for understanding DNA metabolism and as a target for compounds with anticancer or antimicrobial activity.

Our S. cerevisiae Topoisomerase II is prepared by overexpressing the enzyme in yeast and purifying it using proprietary in-house methods that ensure high quality and reproducibility. The enzyme is supplied in dilution buffer and validated for activity and stability. Each batch is supported by stringent quality control testing and comes with full technical documentation.

Enzymatic activity

One Unit of Topo II relaxes 0.5 µg of supercoiled pBR322 DNA in 1X assay buffer in a 30 µl reaction volume at 37°C for 30 minutes. This reliable definition allows straightforward standardisation across experiments and supports reproducible research outcomes.

Kit contents

• Purified S. cerevisiae DNA Topoisomerase II enzyme
• 5X concentrated assay buffer
• Dilution buffer (also available separately)
• Relaxation kits include enzyme, supercoiled DNA substrate, assay buffer, dilution buffer, and 10X GSTEB stop and loading buffer

Applications

Yeast Topoisomerase II is suitable for:

• Relaxation and decatenation assays
• Fundamental research into topoisomerase 2 activity and mechanisms
• Screening of inhibitors targeting topo II and related enzymes
• Comparative studies alongside DNA gyrase and other topoisomerases

Storage and stability

The enzyme should be stored at –80°C for long-term stability. Bulk quantities are available on request for laboratories requiring larger volumes - get in touch

Our yeast topoisomerase II preparations provide robust performance, ensuring dependable results for both academic research and pharmaceutical development.

FAQs about Yeast Topoisomerase II

What is Yeast Topoisomerase II and what does it do?
Yeast DNA Topoisomerase II (also known as topoisomerase II, topoisomerase 2, DNA topoisomerase 2 or topo II) is a type II topoisomerase that regulates DNA topology. It relaxes supercoiled DNA and decatenates intertwined DNA molecules, making it essential for replication, transcription and chromosome segregation.

How is this enzyme produced?
Our yeast topoisomerase II is overexpressed in Saccharomyces cerevisiae and purified using proprietary in-house methods. This ensures high purity, consistency between batches and reliable performance across applications.

What is the enzymatic activity of yeast Topo II?
One Unit of yeast Topoisomerase II relaxes 0.5 µg of supercoiled pBR322 DNA in 1X assay buffer in a 30 µl reaction volume, incubated at 37°C for 30 minutes.

What is included in the kit?
Each enzyme preparation is supplied in dilution buffer, along with 5X concentrated assay buffer. Relaxation kits include the enzyme, supercoiled DNA substrate, assay buffer, dilution buffer and 10X GSTEB stop and loading buffer. Assay Buffer and Dilution buffer can also be ordered separately.

What are the main applications of yeast DNA Topoisomerase II?
Typical applications include:

• Relaxation and decatenation assays
• Mechanistic studies of topoisomerase 2 activity
• Screening of inhibitors targeting DNA topoisomerase II
• Comparative studies with other enzymes such as DNA gyrase and topoisomerase IV

How should the enzyme be stored?
For long-term stability, yeast topoisomerase II should be stored at –80°C. Repeated freeze-thaw cycles should be avoided. Bulk supply is available for laboratories with higher volume requirements.

Do you provide quality control and technical documentation?
Yes. Each batch of yeast topoisomerase II is quality tested and supplied with technical documents containing detailed specifications and lot-specific data.