E. coli Gyrase Supercoiling Assay Kits

These kits are available containing everything required to perform supercoiling reactions and to test inhibitors. They contain the enzyme, relaxed pBR322 DNA substrate, the Assay and Dilution buffers as well as 10X GSTEB - our new stop and loading buffer.

DNA gyrase (gyrase) is a bacterial type II topoisomerase that can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. Escherichia coli DNA gyrase is prepared from the over producing strains JMtacA and JMtacB¹ and is supplied as an A₂B₂ complex.

Store at -80°C.

1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 1X Assay Buffer in a 30 µL reaction volume in 30 minutes at 37°C.

See technical documents for more detailed information and lot specific activities.

Why buy from Inspiralis?

Our easy-to-use kits save you time
Consistent and reliable quality
Fast shipping around the world
Buy online or get an instant quote

Cat no.	Product	Price
K0001	E. coli Gyrase Supercoiling Kit 1 100 U of E. coli Gyrase supplied with 5X Assay Buffer, Dilution Buffer, 50 µg relaxed pBR322 plasmid, and 10X GSTEB	£573.00
K0002	E. coli Gyrase Supercoiling Kit 2 500 U of E. coli Gyrase supplied with 5X Assay Buffer, Dilution Buffer, 250 µg relaxed pBR322 plasmid, and 10X GSTEB	£2,065.00
K003	E. coli Gyrase Supercoiling Kit 3 1000 U of E. coli Gyrase supplied with 5X Assay Buffer, Dilution Buffer, 500 µg relaxed pBR322 plasmid, and 10X GSTEB	£3,692.00
K004	E. coli Gyrase Supercoiling Kit 4 2000 U of E. coli Gyrase supplied with 5X Assay Buffer, Dilution Buffer, 1 mg relaxed pBR322 plasmid, and 10X GSTEB	£6,721.00

Need help? Contact Us

References

Hallett, P., Grimshaw, A.J., Wigley, D.B. and Maxwell, A. (1990) Cloning of the DNA gyrase genes under tac promoter control: overproduction of the gyrase A and B proteins. Gene 93: 139-142