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  • rpBR
  • Substrate Relaxed pBR

Relaxed pBR322 DNA

Relaxed plasmid pBR322 DNA is produced by the large-scale alkaline-lysis method ( Sambrook et al., 1989)1 from a high-copy derivative of pBR322 (Boros et al., 1984)2 and the resulting supercoiled plasmid is relaxed using topoisomerase I. This is further purified and optimised for topoisomerase assays

It is supplied at a concentration of 1mg/ml in TE (10mM Tris-HCl (pH 7.5), 1mM EDTA). Store at 4oC

0.5 µg of relaxed pBR322 when incubated with 1 U of DNA gyrase in a reaction volume of 30 µl at 37oC for 30 minutes in incubation buffer is completely converted to the supercoiled form.

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Cat no. Product Price Quantity
R5001 Relaxed pBR322 Plasmid (50 µg)
50 µg of relaxed pBR322 plasmid DNA
R2502 Relaxed pBR322 Plasmid (250 µg)
250 µg of relaxed pBR322 plasmid DNA
R5003 Relaxed pBR322 Plasmid (500 µg)
500 µg of relaxed pBR322 plasmid DNA
R1004 Relaxed pBR322 Plasmid (1 mg)
1 mg of relaxed pBR322 plasmid DNA
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  1. Sambrook, J., Fritsch, E.F. & Maniatis, T (1989). Molecular cloning: a laboratory manual,2nd ed., Cold Spring Harbor Press. Cold Spring Harbor, NY.
  2. Boros, I., Pósfai, G. & Venetianer, P. (1984). High-copy number derivatives of the plasmid cloning vector pBR322. Gene 30, 257-260
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