M. tuberculosis Gyrase (HIS)
Mycobacterium tuberculosis DNA gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target antibacterial drugs and can be used for screening potential novel antibacterial compounds. It is prepared by overexpressing the subunits with C-terminal polyhistidine tags in E. coli and then purified by affinity chromatography.
It is supplied as an A2B2 complex in Dilution Buffer. Store at -80 °C.
1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 1X Assay Buffer in a 30 µL reaction volume in 30 minutes at 37°C.
All enzyme is supplied with 5X concentrated Assay Buffer and Dilution Buffer which are also available separately.
See technical documents below for more detailed information and lot specific activities.
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