S. pneumoniae Gyrase Supercoiling Assay Kits
These kits are available containing everything required to perform supercoiling reactions and to test inhibitors. They contain the enzyme, relaxed pBR322 DNA substrate, the Assay and Dilution buffers as well as 10X GSTEB - our new stop and loading buffer.
DNA gyrase (gyrase) is a bacterial type II topoisomerase that can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. Streptococcus pneumoniae DNA gyrase is pprepared by overexpressing the subunits in E. coli and then purified by in-house methods.
It is supplied as an A2B2 complex in Dilution Buffer. Store at -80 °C.
1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 1X Assay Buffer in a 30 µL reaction volume in 30 minutes at 37°C.
See technical documents for more detailed information and lot specific activities.
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