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  • Mtub Gyrase Supercoiling

M. tuberculosis Gyrase (HIS) Supercoiling Assay Kits

These kits are available containing everything required to perform supercoiling reactions and to test inhibitors. They contain the enzyme, relaxed pBR322 DNA substrate, the Assay and Dilution buffers as well as 10X GSTEB - our new stop and loading buffer.

Mycobacterium tuberculosis DNA gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target antibacterial drugs and can be used for screening potential novel antibacterial compounds. It is prepared by overexpressing the subunits with C-terminal polyhistidine tags in E. coli and then purified by affinity chromatography.

It is supplied as an A2B2 complex in Dilution Buffer. Store at -80 °C.

1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 1X Assay Buffer in a 30 µL reaction volume in 30 minutes at 37°C.

Please enquire for larger quantities/bulk orders.

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Cat no. Product Price Quantity
MTS001 M. tuberculosis Gyrase (HIS) Supercoiling Kit 1
100 U of M. tuberculosis Gyrase supplied with 5X Assay Buffer, Dilution Buffer, 50 µg relaxed pBR322 plasmid, and 10X GSTEB
£521.00
MTS002 M. tuberculosis Gyrase (HIS) Supercoiling Kit 2
500 U of S. aureus Gyrase supplied with 5X Assay Buffer, Dilution Buffer, 250 µg relaxed pBR322 plasmid, and 10X GSTEB
£1,710.00
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