M. tuberculosis Topoisomerase I
Mycobacterium tuberculosis DNA Topoisomerase I (Topo I) is a bacterial type IA topoisomerase which can relax supercoiled DNA. It is a potential target for antibacterial drugs and can be used for screening potential novel antibacterial compounds. It is prepared by overexpressing the protein in E. coli and then purified by methods developed in-house. The enzyme is supplied in Dilution Buffer.
1 U of topo I will relax 0.5 µg of supercoiled pBR322 DNA in 1X Assay buffer in a total reaction volume of 30 µl in 30 minutes at 37°C.
Store at -80 °C.
The enzyme is supplied with Assay Buffer (5X concentration) and Dilution Buffer (1X) which are also available separately.
See technical documents below for more detailed information and lot specific activities.
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