M. tuberculosis Gyrase (HIS) Decatenation Assay Kits

These kits contain all the components necessary to perform decatenation reactions. Including the enzyme, the catenated kDNA substrate, a decatenated kDNA marker, the Assay and Dilution buffers as well as 10X GSTEB - our new stop and loading buffer.

Mycobacterium tuberculosis DNA gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target antibacterial drugs and can be used for screening potential novel antibacterial compounds. It is prepared by overexpressing the subunits with C-terminal polyhistidine tags in E. coli and then purified by affinity chromatography.

It is supplied as an A₂B₂ complex in Dilution Buffer. Store at -80 °C.

1 U of M. tuberculosis gyrase will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes.

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Cat no.	Product	Price	Quantity
MTD001	M. tuberculosis Gyrase (HIS) Decatenation Assay Kit 1 100 U of M. tuberculosis Gyrase supplied with 5X Assay Buffer, Dilution Buffer, 20 µg kDNA, decatenated kDNA marker and 10X GSTEB	£503.00
MTD002	M. tuberculosis Gyrase (HIS) Decatenation Assay Kit 2 500 U of M. tuberculosis Gyrase supplied with 5X Assay Buffer, Dilution Buffer, 100 µg kDNA, decatenated kDNA marker and 10X GSTEB	£1,662.00