M. tuberculosis Gyrase (HIS) Decatenation Assay Kits
These kits contain all the components necessary to perform decatenation reactions. Including the enzyme, the catenated kDNA substrate, a decatenated kDNA marker, the Assay and Dilution buffers as well as 10X GSTEB - our new stop and loading buffer.
Mycobacterium tuberculosis DNA gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target antibacterial drugs and can be used for screening potential novel antibacterial compounds. It is prepared by overexpressing the subunits with C-terminal polyhistidine tags in E. coli and then purified by affinity chromatography.
It is supplied as an A2B2 complex in Dilution Buffer. Store at -80 °C.
1 U of M. tuberculosis gyrase will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes.
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