Kinetoplast DNA (kDNA)

Kinetoplast catenated DNA (kDNA) is purified from Crithidia fasciculata using the method described in Shapiro (Shapiro et al., 1999)¹ and is supplied at a concentration of 100 ng/µl in 10 mM Tris-HCl (pH8.0), 1 mM EDTA. kDNA is a substrate for use in topo II or topo IV decatenation assays and results can be obtained more quickly than those from relaxation assays because the decatenated mini circles can easily be resolved from the non-decatenated material which remains in the wells of the gel.

Please see He et al. (2023)² and Ramakrishnan et al. (2024)³ for high-resolution, single-molecule studies using our kDNA

Store at 4°C.

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Cat no.	Product	Price
K2001	kDNA (20 µg) 20 µg of kDNA	£191.00
K1002	kDNA (100 µg) 100 µg of kDNA	£693.00
K2003	kDNA (200 µg) 200 µg of kDNA	£1,028.00
K4004	KDNA (400 µg) 400 µg of kDNA	£1,502.00
KD100	Decatenated kDNA Decatenated kDNA marker	£126.00
KL100	Linear kDNA Linear kDNA marker	£126.00

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References

Shapiro, T.A., Klein, V.A. and Englund, P.T. (1999) Isolation of kinetoplast DNA, in DNA Topoisomerase Protocols Vol.I (Bjornsti, M-A and Osheroff, N., eds.). Humana Press Inc., N. Jersey, pp. 61-68
He, P., Katan, A. J., Tubiana, L., Dekker, C., & Michieletto, D. (2023). Single-Molecule Structure and Topology of Kinetoplast DNA Networks. Physical Review X, 13(2), 1-13. Article 021010. https://doi.org/10.1103/PhysRevX.13.021010
Ramakrishnan, S., Chen, Z., Gutierrez Fosado, Y. A., Tubiana, L., Vanderlinden, W., Savill, N. J., Schnaufer, A., & Michieletto, D. (2024). Single-molecule morphology of topologically digested olympic networks. PRX Life.