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  • kDNA
  • Substrate kDNA

Kinetoplast DNA (kDNA)

Kinetoplast catenated DNA (kDNA) is purified from Crithidia fasciculata using the method described in Shapiro (Shapiro et al., 1999)1 and is supplied at a concentration of 100 ng/µl in 10 mM Tris-HCl (pH8.0), 1 mM EDTA. kDNA is a substrate for use in topo II or topo IV decatenation assays and results can be obtained more quickly than those from relaxation assays because the decatenated mini circles can easily be resolved from the non-decatenated material which remains in the wells of the gel.

Please see He et al. (2023)2 and Ramakrishnan et al. (2024)3 for high-resolution, single-molecule studies using our kDNA

Store at 4°C.

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Cat no. Product Price Quantity
K2001 kDNA (20 µg)
20 µg of kDNA
K1002 kDNA (100 µg)
100 µg of kDNA
K2003 kDNA (200 µg)
200 µg of kDNA
K4004 KDNA (400 µg)
400 µg of kDNA
KD100 Decatenated kDNA
Decatenated kDNA marker
KL100 Linear kDNA
Linear kDNA marker
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  1. Shapiro, T.A., Klein, V.A. and Englund, P.T. (1999) Isolation of kinetoplast DNA, in DNA Topoisomerase Protocols Vol.I (Bjornsti, M-A and Osheroff, N., eds.). Humana Press Inc., N. Jersey, pp. 61-68
  2. He, P., Katan, A. J., Tubiana, L., Dekker, C., & Michieletto, D. (2023). Single-Molecule Structure and Topology of Kinetoplast DNA NetworksPhysical Review X13(2), 1-13. Article 021010.
  3. Ramakrishnan, S., Chen, Z., Gutierrez Fosado, Y. A., Tubiana, L., Vanderlinden, W., Savill, N. J., Schnaufer, A., & Michieletto, D. (2024). Single-molecule morphology of topologically digested olympic networks. PRX Life.
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