C. difficile Gyrase

Clostridium difficile DNA gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. It is prepared by over expressing the subunits in E.coli bacteria  and is supplied as an A₂B₂ complex.

Store at -80°C.

This enzyme is supplied with 5X concentrated Assay Buffer and Dilution Buffer which are also available separately.

1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 1X Assay buffer in a total reaction volume of 30 µl in 30 minutes at 37°C.

Please contact us for larger quantities or bulk ordering

See technical documents below for more detailed information and lot specific activities.

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