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E.coli DnaB Unwinding Assay Kits

DnaB is the primary replicative helicase in Escherichia coli and is responsible for unwinding the DNA duplex ahead of the replication fork so that the two strands can be replicated (1). It is a hexameric protein that unwinds DNA in an ATP-dependant manner by translocating along the single-stranded DNA in the 5’ – 3’ direction. The C-terminal domain contains an ATP-binding site. Two DnaB molecules travel in opposite directions along the two DNA strands unwinding the helix and creating two replication forks. DnaB helicase interacts with a number of proteins involved in replication and the principle step in the initiation of replication is the loading of DNA onto DnaB helicase.

The essential role of DnaBs amongst bacterial genomes as well as the structural divergence from their eukaryotic counterparts make bacterial  DnaBs ideal targets for antibiotic development (2).

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Cat no. Product Price Quantity
DNABU01 E.coli DnaB Unwinding Assay Kit - 100 Assays
Unwinding kits contain enough DnaB, 10X assay buffer, dilution buffer, forked substrate and capture oligo for 100 assays along with a 384-well microtitre plate.
£480.00
DNABU05 E.coli DnaB Unwinding Assay Kit - 500 Assays
Unwinding kits contain enough DnaB, 10X assay buffer, dilution buffer, forked substrate and capture oligo for 500 assays along with 384-well microtitre plates.
£1,525.00
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References

(1) West SC. DNA helicases: new breeds of translocating motors and molecular pumps. Cell. 1996, 26;86(2):177-80.

(2) Griep MA, Blood S, Larson MA, Koepsell SA, Hinrichs SH. Myricetin inhibits Escherichia coli DnaB helicase but not primase. Bioorganic & medicinal chemistry. 2007, 15;15(22):7203-8.

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