Human Topoisomerase I Relaxation Assay Kits

• Complete kit for in vitro topoisomerase assays
• Includes enzyme, supercoiled DNA, buffers and stop solution
• Recombinant Human Topoisomerase I produced in insect cells
• 1 Unit relaxes 0.5 µg supercoiled pBR322 DNA in 30 min at 37°C
• Perfect for inhibitor screening and activity studies
• High purity, validated for reproducibility and consistency
• Ready to use with clear, easy-to-follow protocol
• Reliable results for drug discovery and mechanistic research
• Long-term stability when stored at –80°C
• Bulk and custom quantities available on request

Our Human DNA Topoisomerase I Relaxation Assay Kit provides a complete solution for studying topoisomerase activity and inhibition in vitro. Designed for reliability and ease of use, this kit is ideal for researchers working in drug discovery, cancer biology and mechanistic enzymology.

Human DNA Topoisomerase I (Topo I) is a type IB topoisomerase that relaxes supercoiled DNA. The enzyme is produced by overexpression in baculovirus-infected Spodoptera frugiperda insect cells and purified using optimised protocols adapted from Stewart et al., 1996. The enzyme is supplied in Dilution Buffer to maintain stability and performance.

Each DNA topoisomerase assay kit contains all the components required to perform relaxation assays, including:

• Purified Human Topoisomerase I enzyme

• Supercoiled DNA substrate

• Assay and Dilution buffers

• 10X GSTEB stop and loading buffer

Performance

• 1 Unit of Human Topoisomerase I relaxes 0.5 µg of supercoiled pBR322 DNA in 1X Assay Buffer, total reaction volume of 30 µl, incubated for 30 minutes at 37°C.

Applications

• Topoisomerase inhibition assay kit for screening potential drug candidates

• Topoisomerase activity assay kit for fundamental research into DNA topology

• Mechanistic studies of human topoisomerase I

Storage

Store at –80°C for long-term stability.

Bulk supply and larger quantities are available on request. Please contact us to discuss your requirements.

FAQs about our Human Topoisomerase I Relaxation Assay Kits

What is included in the Human Topoisomerase I Relaxation Assay Kit?
Each kit contains purified Human Topoisomerase I enzyme, supercoiled DNA substrate, Assay Buffer, Dilution Buffer and 10X GSTEB stop and loading buffer. These components provide everything required to perform reliable topoisomerase activity assays.

What is Human Topoisomerase I and why is it important?
Human Topoisomerase I is a type IB topoisomerase that relaxes supercoiled DNA. It plays a critical role in DNA replication and transcription, making it a valuable target for drug discovery. Researchers use this DNA topoisomerase assay kit to study enzyme function and to screen for potential inhibitors.

Can this kit be used for inhibitor screening?
Yes. The kit is designed for both activity and inhibition studies. It is commonly used as a topoisomerase inhibition assay kit in drug discovery programmes to identify and characterise compounds that target Human Topoisomerase I.

How much DNA can one unit of enzyme relax?
One unit of Human Topoisomerase I relaxes 0.5 µg of supercoiled pBR322 DNA in 1X Assay Buffer, using a 30 µl reaction volume incubated at 37°C for 30 minutes.

What are the storage conditions for the kit?
The kit should be stored at -80°C to ensure long-term stability and reliable performance.

Do you provide bulk quantities of the Human Topoisomerase I Relaxation Assay Kit?
Yes. Larger quantities and bulk supply are available on request. Please contact us to discuss your requirements.