Human Topoisomerase I Relaxation Assay Kits

These kits contain all the components necessary to perform relaxation assays. Including the enzyme, the supercoiled DNA substrate, the Assay and Dilution buffers as well as 10X GSTEB - our new stop and loading buffer.

Human DNA Topoisomerase I (Topo I) is a type IB topoisomerase which can relax supercoiled DNA. It is prepared by overexpressing the enzyme in baculovirus-infected insect cells (Spodoptera frugiperda) and purifying it by methods adapted from Stewart et al., 1996¹. The enzyme is supplied in Dilution Buffer.

1 U of topo I will relax 0.5 µg supercoiled pBR322 DNA in 1X Assay buffer in a total reaction volume of 30 µl in 30 minutes at 37°C.

Store at -80°C.

Please enquire for larger quantities/bulk orders.

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Cat no.	Product	Price
HTR101	Human Topoisomerase I Relaxation Assay Kit 1 100 U of Human Topo I supplied with 10X Assay Buffer, Dilution Buffer, 50 µg negatively supercoiled pBR322 plasmid, and 10X GSTEB	£432.00
HTR102	Human Topoisomerase I Relaxation Assay Kit 2 500 U of Human Topo I supplied with 10X Assay Buffer, Dilution Buffer, 250 µg negatively supercoiled pBR322 plasmid, and 10X GSTEB	£1,350.00
HTR103	Human Topoisomerase I Relaxation Assay Kit 3 1000 U of Human Topo I supplied with 10X Assay Buffer, Dilution Buffer, 500 µg negatively supercoiled pBR322 plasmid, and 10X GSTEB	£2,339.00
HTR104	Human Topoisomerase I Relaxation Assay Kit 4 2000 U of Human Topo I supplied with 10X Assay Buffer, Dilution Buffer, 1 mg negatively supercoiled pBR322 plasmid, and 10X GSTEB	£4,133.00