P. aeruginosa Topoisomerase IV

• Complete solution for studying DNA decatenation and relaxation using Pseudomonas aeruginosa Topoisomerase IV

• Essential bacterial enzyme involved in DNA replication and chromosome segregation

• Validated antibacterial target for quinolone and coumarin inhibitors – ideal for drug discovery and mechanistic studies

• Purified heterotetrameric C₂E₂ complex produced in E. coli using proprietary in-house purification methods

• Supplied with 5X concentrated Assay Buffer and Dilution Buffer (also available separately)

• 1 Unit relaxes 0.5 µg of supercoiled pBR322 DNA or decatenates 200 ng of kDNA in 30 minutes at 37°C

• Suitable for high-throughput inhibitor screening, decatenation assays, and comparative enzyme studies

• High-purity preparation (>95%), confirmed by SDS-PAGE for reproducible, consistent performance

• Supported by comprehensive technical documentation and lot-specific activity data

• Bulk quantities and custom formats available on request

• Store at –80°C for long-term stability and dependable results

DNA topoisomerase IV is a bacterial type II topoisomerase that plays a key role in maintaining DNA topology. It performs both decatenation and relaxation of supercoiled DNA, processes that are essential for chromosome segregation and replication. Because of this crucial role, topoisomerase 4 is a well-established target for antibacterial compounds, including quinolones and coumarins.

Our Pseudomonas aeruginosa DNA Topoisomerase IV is produced by overexpressing the enzyme subunits in E. coli and purifying them using proprietary in-house methods. The A and B subunits are purified to greater than 95% purity, as confirmed by SDS-PAGE analysis, and supplied as a stable heterotetrameric complex in dilution buffer. Each preparation is quality tested to ensure consistency, reproducibility and high enzymatic performance.

Enzymatic activity

One Unit of Topoisomerase IV relaxes 0.5 µg of supercoiled pBR322 DNA in 1X assay buffer, in a 30 µl total reaction volume at 37°C for 30 minutes. One Unit also decatenates 200 ng of kDNA under the same conditions.

Product format

The enzyme is supplied with:

• Purified P. aeruginosa Topoisomerase IV heterotetramer
• 5X concentrated assay buffer
• Dilution buffer (available separately)

Applications

This enzyme is ideal for:

• Fundamental studies of bacterial DNA topoisomerase 4 function
• Screening inhibitors targeting Pseudomonas aeruginosa topoisomerase IV for antibacterial development
• Mechanistic studies of enzyme activity and inhibitor mode of action
• Comparative studies with other bacterial topoisomerases, including DNA gyrase

Storage and supply

Store at –80°C for long-term stability. Avoid repeated freeze–thaw cycles. Bulk quantities and larger orders are available on request - get in touch

All batches of Pseudomonas aeruginosa DNA topoisomerase IV are prepared under strict quality control and supplied with technical documents, including lot-specific activity data, to support reliable and reproducible research.

FAQs About P. aeruginosa Topoisomerase IV

What is Pseudomonas aeruginosa DNA Topoisomerase IV and what does it do?
DNA Topoisomerase IV (Topo IV) is a bacterial type II topoisomerase that regulates DNA topology. It performs both decatenation and relaxation of supercoiled DNA, which are essential for chromosome segregation and replication. In Pseudomonas aeruginosa, topoisomerase 4 is a validated antibacterial target, inhibited by quinolones and coumarins.

How is P. aeruginosa Topoisomerase IV produced?
The enzyme is produced by overexpressing the ParC and ParE subunits in E. coli and purifying them using proprietary in-house methods. The subunits are assembled into a stable heterotetrameric complex and purified to greater than 95% purity, as verified by SDS-PAGE.

What is the enzymatic activity of this enzyme?
One Unit of P. aeruginosa Topoisomerase IV relaxes 0.5 µg of supercoiled pBR322 DNA in 1X assay buffer, in a 30 µl reaction at 37°C for 30 minutes. One Unit also decatenates 200 ng of kDNA under the same conditions.

What is included in the product?
Each enzyme preparation is supplied in dilution buffer, together with a 5X concentrated assay buffer. Additional dilution buffer can also be ordered separately.

What are the main applications of P. aeruginosa DNA Topoisomerase IV?
Typical uses include:

• Studies of DNA topology and enzyme mechanism
• Screening inhibitors of topoisomerase 4 for antibacterial drug discovery
• Comparative research with DNA gyrase and other topoisomerases
• Mechanistic analysis using relaxation or decatenation assays

How should the enzyme be stored?
Store at –80°C for optimal long-term stability. Avoid repeated freeze–thaw cycles. Bulk quantities and large-scale preparations are available on request.

Do you provide documentation and technical support?
Yes. Each batch of Pseudomonas aeruginosa Topoisomerase IV is supplied with detailed technical documentation and lot-specific activity data. Our scientific team is available to advise on assay design, troubleshooting and custom requirements.