S. aureus Gyrase Supercoiling Assay Kits

These kits contain everything required to perform supercoiling reactions and to test inhibitors. They contain the enzyme, relaxed pBR322 DNA substrate, the Assay and Dilution buffers as well as 10X GSTEB - our new stop and loading buffer.

Staphylococcus aureus DNA gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. It is prepared by overexpressing the subunits in E. coli and then purified.

It is supplied as an A₂B₂ complex in Dilution Buffer. Store at -80 °C.

5X concentrated Assay Buffer and Dilution Buffer are also available separately.

1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 1X Assay Buffer in a 30 µL reaction volume in 30 minutes at 37°C.

Please enquire for larger quantities/bulk orders.

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Cat no.	Product	Price	Quantity
SAS4001	S. aureus Gyrase Supercoiling Assay Kit 1 100 U of S. aureus Gyrase supplied with 5X Assay Buffer, Dilution Buffer, 50 µg relaxed pBR322 plasmid, and 10X GSTEB	£542.00
SAS4002	S. aureus Gyrase Supercoiling Assay Kit 2 500 U of S. aureus Gyrase supplied with 5X Assay Buffer, Dilution Buffer, 250 µg relaxed pBR322 plasmid, and 10X GSTEB	£1,929.00