High / Medium-Throughput Assay Kit - E. coli Gyrase

The kit is supplied with sufficient E. coli gyrase enzyme, relaxed pNO1 DNA substrate, buffers and other assay components for 100 assays¹ including a black streptavidin 96-well plate. The enzyme is supplied at a minimum concentration of 5 U/μl in Dilution Buffer ². The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use.

Prices are per kit.

Discounts are available for larger quantities. Please contact us for more information.

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References

Maxwell, A., Burton, N.P. and O'Hagan, N. (2006) High-throughput assays for DNA gyrase and other topoisomerases. Nucleic Acid Res. 34 (15), e104
Hallett, P., Grimshaw, A.J., Wigley, D.B. and Maxwell, A. (1990) Cloning of the DNA gyrase genes under tac promoter control: overproduction of the gyrase A and B proteins. Gene 93: 139-142