Human Topoisomerase II Alpha Decatenation Assay Kits

These kits contain all the components necessary to perform decatenation reactions. Including the enzyme, the catenated kDNA substrate, a decatenated kDNA marker, the Assay and Dilution buffers as well as 10X GSTEB - our new stop and loading buffer.

Human DNA Topoisomerase II alpha (Topo IIa) is a type II topoisomerase that can decatenate and relax supercoiled DNA. It is prepared by overexpressing in baculovirus-infected insect cells (Spodoptera frugiperda) and purifying it by methods developed in-house.

The enzyme is supplied in Dilution Buffer.

1 U of topo IV will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes.

Please enquire for larger quantities/bulk orders.

See technical documents below for more detailed information and lot specific activities.

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Cat no.	Product	Price
HTK201	Human Topoisomerase II Alpha Decatenation Kit 1 Contains 100 U of human topo II alpha, 20 µg kDNA, ATP, Assay Buffer, Dilution Buffer, decatenated kDNA marker and 10X GSTEB	£419.00
HTK202	Human Topoisomerase II Alpha Decatenation Kit 2 Contains 500 U of human topo II alpha, 100 µg kDNA, ATP, Assay Buffer, Dilution Buffer, decatenated kDNA marker and 10X GSTEB	£1,343.00
HTK203	Human Topoisomerase II Alpha Decatenation Kit 3 Contains 1000 U of human topo II alpha, 200 µg kDNA, ATP, Assay Buffer, Dilution Buffer, decatenated kDNA marker and 10X GSTEB	£2,042.00
HTK204	Human Topoisomerase II Alpha Decatenation Kit 4 Contains 2000 U of human topo II alpha, 400 µg kDNA, ATP, Assay Buffer, Dilution Buffer, decatenated kDNA marker and 10X GSTEB	£3,003.00