E. coli Gyrase

DNA gyrase (gyrase) is a bacterial type II topoisomerase that can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. Escherichia coli DNA gyrase is prepared from the over producing strains JMtacA and JMtacB¹ and is supplied as an A₂B₂ complex.

Store at -80°C.

This enzyme is supplied with 5X concentrated Assay Buffer and Dilution Buffer which are also available separately.

1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 1X Assay Buffer in a 30 µL reaction volume in 30 minutes at 37°C.

The low-endotoxin DNA gyrase and GLP-grade DNA gyrase are available on request. For more information about specifications please contact us ([email protected]).

Please enquire for larger quantities/bulk orders.

See technical documents for more detailed information and lot specific activities.

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References

1. Hallett, P., Grimshaw, A.J., Wigley, D.B. and Maxwell, A. (1990) Cloning of the DNA gyrase genes under tac promoter control: overproduction of the gyrase A and B proteins. Gene 93: 139-142