E. coli Gyrase
DNA gyrase (gyrase) is a bacterial type II topoisomerase that can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. Escherichia coli DNA gyrase is prepared from the over producing strains JMtacA and JMtacB1 and is supplied as an A2B2 complex.
Store at -80°C.
This enzyme is supplied with 5X concentrated Assay Buffer and Dilution Buffer which are also available separately.
1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 1X Assay Buffer in a 30 µL reaction volume in 30 minutes at 37°C.
The low-endotoxin DNA gyrase and GLP-grade DNA gyrase are available on request. For more information about specifications please contact us ([email protected]).
Please enquire for larger quantities/bulk orders.
See technical documents for more detailed information and lot specific activities.
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- Hallett, P., Grimshaw, A.J., Wigley, D.B. and Maxwell, A. (1990) Cloning of the DNA gyrase genes under tac promoter control: overproduction of the gyrase A and B proteins. Gene 93: 139-142