E. coli Gyrase

• Complete solution for studying DNA supercoiling and the topoisomerase II, DNA gyrase activity
• Essential bacterial enzyme introducing negative supercoils into DNA for replication and transcription studies
• Primary target for quinolone and coumarin antibiotics - ideal for antibacterial drug discovery and inhibitor screening
• Supplied as a purified heterotetrameric A₂B₂ complex from proprietary E. coli strains
• Includes 5X concentrated Assay Buffer and Dilution Buffer (available separately)
• 1 Unit supercoils 0.5 µg of relaxed pBR322 DNA in 30 minutes at 37°C
• Perfect for ATPase assay kit formats, supercoiling inhibition assays, and mechanistic studies
• Available as Low-Endotoxin and Animal-Free Origin preparations for regulated or sensitive studies
• High-quality, reproducible performance with full technical documentation included
• Bulk quantities and custom supply available on request
• Store at -80°C for long-term stability and reliable results

DNA gyrase in E. coli is a bacterial type II topoisomerase that introduces negative supercoils into DNA. This essential enzyme, also known as bacterial topoisomerase gyrase or topoisomerase II DNA gyrase, plays a central role in DNA replication and transcription. Because of its crucial function, it is a primary target for quinolone and coumarin antibiotics, making it an important tool for antibacterial research and inhibitor screening.

The E. coli DNA gyrase enzyme is prepared from proprietary overproducing strains and is supplied as a heterotetrameric A2B2 complex. Each preparation is supported by detailed technical documentation, ensuring high quality and reproducible results.

Kit contents

• Purified E. coli DNA gyrase (A2B2 complex)
• 5X concentrated Assay Buffer
• Dilution Buffer (also available separately)

Performance

1 Unit of gyrase supercoils 0.5 µg of relaxed pBR322 DNA in 1X Assay Buffer, in a 30 µl reaction volume, incubated at 37°C for 30 minutes.

Applications

• Studying topoisomerase II gyrase activity in bacterial systems
• Screening inhibitors with an ATPase assay kit approach or by inhibition of Supercoiling Assay.
• Antibacterial drug discovery programmes
• Mechanistic and biochemical studies of DNA topology
• Underwinding of circular DNA for Gene Therapy Applications

Options and supply

• Low-endotoxin DNA gyrase available on request
• Animal-Free Origin DNA gyrase available for regulated studies
• Bulk quantities supplied on enquiry

Storage

Store at –80°C to maintain stability and performance.

Please contact us for further information, detailed specifications or to discuss larger order requirements.

FAQs about E. coli Gyrase

What is DNA gyrase in E. coli?
DNA gyrase is a bacterial topoisomerase gyrase found in Escherichia coli. It is a type II topoisomerase that introduces negative supercoils into DNA, a process essential for replication, transcription and chromosome maintenance.

Why is E. coli DNA gyrase important in research?
Because of its central role in DNA topology, topoisomerase II DNA gyrase is a major target for antibacterial agents such as quinolone and coumarin drugs. This makes it a valuable enzyme for both fundamental research and drug discovery programmes.

How is E. coli DNA gyrase supplied?
Our enzyme is prepared from proprietary overproducing E. coli strains and is supplied as a heterotetrameric A2B2 complex. Each kit includes purified enzyme, 5X concentrated Assay Buffer and Dilution Buffer, with buffers also available separately.

Can this enzyme be used in an ATPase assay kit format?
Yes. DNA gyrase activity requires ATP, and the enzyme is frequently studied using ATPase assay kit approaches to measure inhibitor effects or mechanistic activity.

How much DNA can one unit of E. coli DNA gyrase supercoil?
One unit of enzyme supercoils 0.5 µg of relaxed pBR322 DNA in 1X Assay Buffer, in a 30 µl reaction volume, incubated at 37°C for 30 minutes.

Do you provide low-endotoxin or Animal-Free Origin DNA gyrase?
Yes. Both low-endotoxin and GLP-gradeAnimal-Free Origin topoisomerase II gyrase preparations are available on request to meet specific research or regulatory requirements.

What are the recommended storage conditions?
The enzyme should be stored at –80°C to ensure long-term stability and reproducible activity. Dilution of the enzyme before storage is not recommended.

Can I order bulk quantities of bacterial topoisomerase gyrase?
Yes. Larger quantities and bulk supply are available. Please contact us to discuss your project needs.

References

1. Hallett, P., Grimshaw, A.J., Wigley, D.B. and Maxwell, A. (1990) Cloning of the DNA gyrase genes under tac promoter control: overproduction of the gyrase A and B proteins. Gene 93: 139-142