S. aureus Topoisomerase IV

• Complete solution for studying bacterial DNA topology in vitro
• S. aureus Topoisomerase IV (Topo IV) - a type II topoisomerase essential for DNA replication and chromosome segregation
• Performs both decatenation and relaxation of supercoiled DNA
• Produced by overexpressing enzyme subunits in E. coli and purified using proprietary in-house methods
• Supplied as a >95% pure heterotetrameric complex, confirmed by SDS-PAGE
• Includes purified enzyme, 5X Assay Buffer, and Dilution Buffer (available separately)
• 1 Unit relaxes 0.5 µg supercoiled pBR322 DNA or decatenates 200 ng kDNA in 30 minutes at 37 °C
• Ideal for inhibitor screening, ATPase kit formats, and comparative enzyme studies
• Trusted for antibacterial drug discovery and mechanistic research
• High-quality, reproducible performance validated through strict QC
• Store at -80 °C for long-term stability and reliable activity
• Bulk and custom quantities available for academic or pharmaceutical use

Topoisomerase IV (Topo IV) is a bacterial type II topoisomerase that plays a central role in DNA topology. It is responsible for both decatenation and relaxation of supercoiled DNA, processes that are essential for DNA replication and chromosome segregation. Because of its vital cellular function, DNA topoisomerase IV is a validated target for antibacterial compounds, including the clinically important quinolones and coumarins.

Our S. aureus Topoisomerase IV is produced by overexpressing the enzyme subunits in E. coli and purifying them using proprietary methods developed in-house. The subunits are purified to greater than 95% purity, as confirmed by SDS-PAGE, and supplied as a heterotetramer complex in Dilution Buffer.

Kit contents

• Purified S. aureus Topoisomerase IV heterotetramer
• 5X concentrated Assay Buffer
• Dilution Buffer (also available separately)
• Enzymatic performance
• 1 Unit of Topo IV relaxes 0.5 µg of supercoiled pBR322 DNA in 1X Assay Buffer, in a total reaction volume of 30 µl, incubated at 37°C for 30 minutes.
• 1 Unit of Topo IV decatenates 200 ng of kDNA under the same conditions.

Applications

• Fundamental studies of topoisomerase IV and its role in bacterial DNA replication
• Screening inhibitors of topoisomerase 4 for antibacterial drug discovery
• Mechanistic investigations using an ATPase kit format
• Comparative studies of bacterial topoisomerase II enzymes, including DNA gyrase and Topo IV

Storage and supply

The enzyme should be stored at –80°C for long-term stability and reliable performance. Bulk orders and larger quantities are available on request - get in touch

Our DNA topoisomerase 4 preparations are produced under stringent quality standards to ensure reproducibility and accuracy, supporting both academic research and pharmaceutical development. For further information and lot-specific data, please consult the technical documents provided.

FAQs about S. aureus Topoisomerase IV

What is S. aureus Topoisomerase IV and why is it important?
Topoisomerase IV (also known as DNA topoisomerase IV or DNA topoisomerase 4) is a bacterial type II topoisomerase that regulates DNA topology. It carries out essential functions such as decatenation and relaxation of supercoiled DNA during replication and chromosome segregation. Because of its central role, topoisomerase IV is a validated antibacterial drug target and is inhibited by compounds such as quinolones and coumarins.

What is included in the Topoisomerase IV kit?
Each kit contains purified S. aureus Topoisomerase IV heterotetramer, 5X concentrated assay buffer, and dilution buffer. Additional assay buffer and l dilution buffer can be ordered separately.

How is the enzyme produced and what is its purity?
Our S. aureus Topoisomerase IV is produced by overexpressing both subunits in E. coli. The proteins are then purified using proprietary in-house methods to greater than 95% purity, confirmed by SDS-PAGE. The subunits are supplied as a stable heterotetrameric complex in dilution buffer.

How is enzymatic activity defined for this product?
One Unit of Topoisomerase IV relaxes 0.5 µg of supercoiled pBR322 DNA in a 30 µl reaction volume at 37°C in 30 minutes using 1X assay buffer. One Unit also decatenates 200 ng of kDNA under the same conditions.

What are the main applications of this enzyme?
Applications include:

• Fundamental studies of bacterial DNA topoisomerase IV function
• Screening of topoisomerase 4 inhibitors for antibacterial discovery programmes
• Mechanistic studies using an ATPase kit format
• Comparative investigations of bacterial type II topoisomerases, such as DNA gyrase and Topo IV

How should the enzyme be stored?
For long-term stability and reproducibility, store S. aureus Topoisomerase IV at –80°C. Avoid repeated freeze–thaw cycles. Bulk quantities are available on request for laboratories requiring larger supplies.

Is technical support available for assay set-up and optimisation?
Yes. Our scientific team is available to provide advice on experimental design, assay conditions, and troubleshooting. We can also discuss custom formats and bulk orders to suit your research requirements - get in touch

Do you provide lot-specific data and documentation?
Yes. Each preparation of DNA topoisomerase IV is supplied with technical documents and quality control information to ensure consistent performance across batches.