C. difficile Gyrase Supercoiling Assay Kits
These kits are available containing everything required to perform supercoiling reactions and to test inhibitors. They contain the enzyme, relaxed pBR322 DNA substrate, the Assay and Dilution buffers as well as 10X GSTEB - our new stop and loading buffer.
Clostridium difficile DNA gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. It is prepared by over expressing the subunits in E.coli bacteria and is supplied as an A2B2 complex.
It is supplied as an A2B2 complex in Dilution Buffer. Store at -80 °C.
5X concentrated Assay Buffer and Dilution Buffer are also available separately.
1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 1X Assay Buffer in a 30 µL reaction volume in 30 minutes at 37°C.
Please enquire for larger quantities/bulk orders.
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