Supercoiled pBR322 DNA
Supercoiled plasmid pBR322 DNA is produced by the large-scale alkaline-lysis method (Sambrook et al., 1989)1 from a high-copy derivative of pBR322 (Boros et al., 1984)2.
Negatively-supercoiled pBR322 DNA is further treated so that the DNA is more supercoiled and has a narrow range of linking numbers making it an ideal substrate for relaxation reactions.
Positively-supercoiled plasmid pBR322 DNA is produced by treating relaxed pBR322 with reverse gyrase before final purification.
It is supplied at a concentration of 1mg/ml in TE (10mM Tris-HCl (pH 7.5), 1mM EDTA). Store at 4oC
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References
- Sambrook, J., Fritsch, E.F. & Maniatis, T (1989). Molecular cloning: a laboratory manual,2nd ed., Cold Spring Harbor Press. Cold Spring Harbor, NY.
- Boros, I., Pósfai, G. & Venetianer, P. (1984). High-copy number derivatives of the plasmid cloning vector pBR322. Gene 30, 257-260
Technical Documents
Data Sheets
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