A. baumannii Topoisomerase IV

Topo IV (from Acinetobacter baumannii) is prepared by overexpressing the subunits in E. coli and purifying them by methods developed in-house.

The subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex  in Dilution buffer.

1 U of topo IV will relax 0.5 µg supercoiled pBR322 DNA in 1X Assay buffer in a total reaction volume of 30 µl in 30 minutes at 37°C.

1 U of topo IV will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl in 30 minutes at 37°C. 

Store at -80ºC.

All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.

See technical documents below for more detailed information and lot specific activities.

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