P. aeruginosa Gyrase Supercoiling Assay Kits

These kits are available containing everything required to perform supercoiling reactions and to test inhibitors. They contain the enzyme, relaxed pBR322 DNA substrate, the Assay and Dilution buffers as well as 10X GSTEB - our new stop and loading buffer.

Pseudomonas aeruginosa DNA gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. It is prepared by overexpressing the subunits in E. coli and then purified.

Store at -80°C.

1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 1X Assay Buffer in a 30 µL reaction volume in 30 minutes at 37°C.

See technical documents for more detailed information and lot specific activities.

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Cat no.	Product	Price	Quantity
PAS001	P. aeruginosa Gyrase Supercoiling Kit 1 100 U of P. aeruginosa Gyrase supplied with 5X Assay Buffer, Dilution Buffer, 50 µg relaxed pBR322 plasmid, and 10X GSTEB	£537.00
PAS002	P. aeruginosa Gyrase Supercoiling Kit 2 500 U of P. aeruginosa Gyrase supplied with 5X Assay Buffer, Dilution Buffer, 250 µg relaxed pBR322 plasmid, and 10X GSTEB	£1,914.00