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  • Yeast Topo II relax

Yeast Topoisomerase II

Yeast (Saccharomyces cerevisae) DNA Topoisomerase II (Topo II) is a type II topoisomerase which can relax and decatenate DNA. It is prepared by overexpressing in Saccharomyces cerevisae and purifying it by methods developed in-house. The enzyme is supplied in Dilution Buffer.

1 U of topo II will relax 0.5 µg supercoiled pBR322 DNA in 1X Assay buffer in a total reaction volume of 30 µl in 30 minutes at 37°C.

This enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffer which are also available separately.

The relaxation kits contain the enzyme, the supercoiled DNA substrate, the Assay and Dilution buffers as well as 10X GSTEB - our new stop and loading buffer.

Store at -80°C.

See technical documents below for more detailed information and lot specific activities.

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Cat no. Product Price Quantity
YT201 Yeast Topoisomerase II (100 U)
100 U of Yeast Topo II supplied with 5X Assay Buffer and Dilution Buffer
£351.00
YT205 Yeast Topoisomerase II (500 U)
500 U of Yeast Topo II supplied with 5X Assay Buffer and Dilution Buffer
£1,298.00
YTR201 Yeast Topoisomerase II Relaxation Assay Kit 1
100 U of Yeast Topo II supplied with 50 µg negatively supercoiled pBR322, 5X Assay Buffer, Dilution Buffer, and 10X GSTEB
£554.00
YTR205 Yeast Topoisomerase II Relaxation Assay Kit 2
500 U of Yeast Topo II supplied with 250 µg negatively supercoiled pBR322, 5X Assay Buffer, Dilution Buffer, and 10X GSTEB
£2,034.00
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