Biochemical Assays

Our assay kits include everything required to test for inhibitors, however, Inspiralis can save you further time and resources by running the assays on your behalf, or providing custom screening services, delivering accurate interpretation and insights as a research collaborator. 

Our team has many years of in-depth experience in the field of modifying enzymes, and we will combine this expertise with our high-quality kits to produce accurate, reliable results on which you can build your research. 

We’re a responsive research partner, with the ability to work to short turnaround times while never compromising on quality or scientific process. The resulting data is owned exclusively by you and will never be shared - all research contracts feature an NDA and MSA. Please contact us or complete the form below to discuss your project.

Fragment Screening

We are able to offer inhibition assays for any of our products. we can assist with hit identification, determination of IC_⁵⁰ values or investigation into the mechanism of action.

Any number or combination of assays can be carried out and there is no minimum number of compounds required.

All assays are performed in-house by experienced researchers. Results are provided as a full written report containing original gel images or raw data, processed data and subsequent statistical analysis. We have many years of experience working with these types of assays and can quickly identify positive inhibitors saving you time and money. The data ownership is yours and will never be shared; confidentiality is paramount.

Just send us your compounds and we will do the rest.

Topoisomerase Assays

We offer a number of topoisomerase assays which can identify inhibitors, determine IC_⁵⁰ values or investigate the mechanism of action. They include a medium/high-throughput plate-based assay, gel-based supercoiling, relaxation and decatenation reactions, ATPase assays, cleavage assays and DNA unwinding assays.

ATPase Assays

Hydrolysis of ATP is essential for the activity of topoisomerase II enzymes as well as many other DNA modifying enzymes and inhibition of the ATPase activity is the mechanism by which compounds such as coumarins (e.g. novobiocin) inhibit the enzymes. With DNA topoisomerases the activity of the complete enzyme is stimulated by binding DNA (the E.coli GyrB43 domain is not stimulated). The reaction can be followed by a number of methods. In the linked assay, the hydrolysis of ATP is linked to the conversion of NADH to NAD+ which can then be measured by a change in absorbance at 340nm. This is a continuous assay which allows the course of the reaction to be monitored. Details of the assays can be found here. An alternative assay which uses Malachite Green to directly measure the phosphate produced by the hydrolysis of ATP to ADP is an end-point assay. We are also able to supply this assay if you prefer. Please enquire for more information.

Pricing

We offer competitive pricing and can offer discounts for larger projects. 

You can send us your requirements using the form below, or contact us directly on +44 (0) 1603 673591 or [email protected]