S. pneumoniae Gyrase

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S. pneumoniae Gyrase

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Streptococcus pneumoniae gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. It is prepared by overexpressing the subunits in E. coli and then purified.

It is supplied as an A2B2 complex in Dilution Buffer. Store at -80 °C.

All enzyme is supplied with 5X concentrated Assay Buffer and Dilution Buffer which are also available separately. 1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 30 minutes at 37 °C.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No. Product Price Quantity
SPG1001 S. pneumoniae Gyrase
100U
£105
SPG1002 S. pneumoniae Gyrase
500U
£386
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S. pneumoniae Gyrase Supercoiling Assay Kits

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Kits are available containing everything required to perform supercoiling reactions and to test inhibitors. They contain the enzyme, relaxed DNA substrate and the Assay and Dilution buffers. 

Technical Documents

Cat No. Product Price Quantity
SPS001 S.pneumoniae Gyrase Supercoiling Assay Kit 1
100U S. pneumoniae Gyrase and 50ug relaxed pBR322
£184
SPS002 S.pneumoniae Gyrase Supercoiling Assay Kit 2
500U S. pneumoniae Gyrase and 50ug relaxed pBR322
£689
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