P. aeruginosa Gyrase

Click on the product headings below to view details and order online.

P. aeruginosa Gyrase

>

Pseudomonas aeruginosa gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. It is prepared by overexpressing the subunits in E. coli and then purified.

It is supplied as an A2B2 complex in Dilution Buffer. Store at -80 °C.

All enzyme is supplied with 5X concentrated Assay Buffer and Dilution Buffer which are also available separately. 1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 30 minutes at 37 °C.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No. Product Price Quantity
PAG1001 P. aeruginosa Gyrase
100 U
£105
PAG5002 P. aeruginosa Gyrase
500 U
£382
Custom Quote

P. aeruginosa Gyrase Supercoiling Assay Kits

>

Kits are available containing everything required to perform supercoiling reactions and to test inhibitors. They contain the enzyme, relaxed DNA substrate and the Assay and Dilution buffers. 

Technical Documents

Cat No. Product Price Quantity
PAS001 P.aeruginosa Gyrase Supercoiling Assay Kit 1
100 U of P. aeruginosa gyrase and 50 µg of relaxed DNA
£184
PAS002 P.aeruginosa Gyrase Supercoiling Kit 2
500 U of P. aeruginosa gyrase and 50 µg of relaxed DNA
£689
Custom Quote