P. aeruginosa Topoisomerase IV

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P. aeruginosa Topo IV

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Pseudomonas aeruginosa topo IV is prepared by overexpressing the subunits in E. coli and purifying them by methods developed in-house.

The subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex  in Dilution buffer.

It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles. Store at -80ºC.

All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No. Product Price Quantity
PAT4001 P. aeruginosa Topo IV
100 U
£130
PAT4002 P. aeruginosa Topo IV
500 U
£475
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P. aeruginosa Topo IV Decatenation Assay Kits

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These contain the enzyme and the catenated kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of topo IV will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes. 

Technical Documents

Cat No. Product Price Quantity
PAD4001 P. aeruginosa Topo IV Decatenation Kit 1
100 U of P. aeruginosa topo IV enzyme and 20 µg kDNA
£210
PAD4002 P. aeruginosa Topo IV Decatenation Kit 2
500 U of P. aeruginosa Topo IV and 100 µg kDNA
£778
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