Human Topoisomerase II alpha and beta

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Human Topoisomerase II Alpha

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Human topoisomerase II alpha is prepared by overexpressing in baculovirus-infected insect cells (Spodoptera frugiperda) and purifying it by methods developed in-house.

The enzyme is supplied in Dilution Buffer.

Store at -80°C.

It is recommended that larger pack sizes (500U and above) of the enzyme are aliquoted to avoid repeated freeze-thaw cycles.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No. Product Price Quantity
HT201 Human Topo II alpha
100 U
£68
HT205 Human Topo II alpha
500 U
£215
HT210 Human Topo II alpha
1000 U
£365
HT220 Human Topo II alpha
2000 U
£608
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Human Topoisomerase II Beta

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Human topoisomerase II beta is prepared by overexpressing in baculovirus-infected insect cells (Spodoptera frugiperda) and purifying it by methods developed in-house.

The enzyme is supplied at a concentration of 2-10 U/μl in Dilution Buffer.

Store at -80°C. (Stable for 3 months undiluted).

It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles.

Technical Documents

Cat No. Product Price Quantity
HTB201 Human Topo II beta
100U
£68
HTB205 Human Topo II beta
500U
£215
HTB210 Human Topo II beta
1000U
£365
HTB220 Human Topo II beta
2000U
£608
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Human Topoisomerase II Decatenation Assay Kits

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These contain human topo II and the catenated kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of human topo II will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes. The kits are available with either the alpha or beta forms of the enzyme. 

Technical Documents

Cat No. Product Price Quantity
HTK201 Human Topo II alpha Decatenation Assay Kit 1
Contains 100 U of human topo II alpha and 20 µg kDNA
£165
HTK202 Human Topo II alpha Decatenation Assay Kit 2
Contains 500 U of human topo II alpha and 100 µg kDNA
£580
HTK203 Human Topo II alpha Decatenation Assay Kit 3
Contains 1000 U of human topo II alpha and 200 µg kDNA
£924
HTK204 Human Topo II alpha Decatenation Assay Kit 4
Contains 2000 U of human topo II alpha and 400 µg kDNA
£1,467
HTKB201 Human Topo II beta Decatenation Assay Kit 1
Contains 100 U of human topo II beta and 20 µg kDNA
£165
HTKB202 Human Topo II beta Decatenation Assay Kit 2
Contains 500 U of human topo II beta and 100 µg kDNA
£580
HTKB203 Human Topo II beta Decatenation Assay Kit 3
Contains 1000 U of human topo II beta and 200 µg kDNA
£924
HTKB204 Human Topo II beta Decatenation Assay Kit 4
Contains 2000 U of human topo II beta and 400 µg kDNA
£1,467
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Human Topoisomerase II Relaxation Assay Kits

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These contain human topo II and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of human topo II will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C.  The kits are available with either the alpha or beta forms of the enzyme. 

Technical Documents

Cat No. Product Price Quantity
HTR201 Human Topo II alpha Relaxation Assay Kit 1
Contains 100 U of human topo II alpha and 50 µg supercoiled DNA
£165
HTR202 Human Topo II alpha Relaxation Assay Kit 2
Contains 500 U of human topo II alpha and 250 µg supercoiled DNA
£580
HTR203 Human Topo II alpha Relaxation Assay Kit 3
Contains 1000 U of human topo II alpha and 500 µg supercoiled DNA
£1,086
HTR204 Human Topo II alpha Relaxation Assay Kit 4
Contains 2000 U of human topo II alpha and 1 mg supercoiled DNA
£1,680
HTRB201 Human Topo II beta Relaxation Assay Kit 1
Contains 100 U of human topo II beta and 50ug supercoiled DNA
£165
HTRB202 Human Topo II beta Relaxation Assay Kit 2
Contains 500 U of human topo II beta and 250 µg supercoiled DNA
£580
HTRB203 Human Topo II beta Relaxation Assay Kit 3
Contains 1000 U of human topo II beta and 500 µg supercoiled DNA
£1,086
HTRB204 Human Topo II beta Relaxation Assay Kit 4
Contains 2000 U of human topo II beta and 1 mg supercoiled DNA
£1,680
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Human Topoisomerase II Assay Kits For Cell Extracts

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These kits are designed for assaying cell extracts and partially purified fractions containing human topo II. They contain kDNA substrate, Assay buffer, Dilution buffer, decatenated and linear DNA markers and stop buffer / loading dye.

The kit components are based on the standard assay which contains 500 ng substrate DNA per assay.

Technical Documents

Cat No. Product Price Quantity
HDAK1 Kit 1
Contains sufficient kDNA, assay and dilution buffers for 100 assays as well as linear and decatenated marker DNA
£156
HDAK2 Kit 2
Contains linear and decatenated marker DNA
£390
HDAK3 Kit 3
Contains sufficient kDNA, assay and dilution buffers for 500 assays as well as linear and decatenated marker DNA
£500
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High / Medium-Throughput Assay Kit - Human Topoisomerase II

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The kit is supplied with sufficient human topo II enzyme, plasmid DNA substrate, buffers and other assay components* for 100 assays. The enzyme is supplied at a concentration of 10 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use.  The kits are available with either the alpha or beta forms of the enzyme. 

More information about this assay can be found on the 'Services' page under 'High/Medium Throughput Assay'.

Kit issued with limited licence for individual use only. 

Patent held by Inspiralis Ltd., Norwich, Norfolk, UK. (Patent No. GB0424953.8, US7838230)

Technical Documents

Cat No. Product Price Quantity
TRT201 HTS Assay Kit - Human Topo II 1-5 Kits
For 100 assays per plate
£410
TRT202 HTS Assay Kit - Human Topo II 6+ Kits
For 100 assays per plate
£330
TRTB201 HTS Assay Kit - Human Topo II Beta 1-5 Kits
For 100 assays per plate
£410
TRTB202 HTS Assay Kit - Human Topo II Beta 6+ Kits
For 100 assays per plate
£330
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Human Topoisomerase II alpha 453 subunit

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Human topoisomerase II alpha amino acids 1-453. This contains the ATPase domain, the activity of which can be stimulated by DNA (see Campbell and Maxwell (2002), J.Biol.Chem. 320 p.171.  The protein is expressed in E.coli.

Technical Documents

Cat No. Product Price Quantity
HT453 Human Topo II 453 domain
Please enquire
POA
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