A. baumannii Topo IV

Click on the product headings below to view details and order online.

A. baumannii Topoisomerase IV

>

Topo IV (from Acinetobacter baumannii) is prepared by overexpressing the subunits in E. coli and purifying them by methods developed in-house.

The subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex  in Dilution buffer.

It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles. Store at -80ºC.

All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No. Product Price Quantity
ABT4001 A. baumannii Topo IV
100U
£127
ABT4002 A. baumannii Topo IV
500U
£475
Custom Quote

A. baumannii Topoisomerase IV Relaxation Assay Kits

>

These contain A. baumannii topo IV and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of topo IV will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C.  

Technical Documents

Cat No. Product Price Quantity
ABR001 A.baumannii Topo IV Relaxation Kit 1
100U for 100 assays
£210
ABR002 A.baumannii Topo IV Relaxation Kit 2
500U for 100 assays
£778
Custom Quote

A. baumannii Topoisomerase IV Decatenation Assay Kits

>

These contain A. baumannii topo IV and the catenated kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of topo IV will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes.  

Technical Documents

Cat No. Product Price Quantity
ABD001 A.baumannii Topo IV Decatenation Kit 1
100U for 100 assays
£210
ABD002 A.baumannii Topo IV Decatenation Kit 2
500U for 100 assays
£778
Custom Quote