A. baumannii Gyrase

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A. baumannii Gyrase

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Acinetobacter baumannii gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. Gyrase is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. It is prepared by overexpressing the subunits in E. coli and then purified.

It is supplied as an A2B2 complex in Dilution Buffer. Store at -80 °C.

All enzyme is supplied with 5X concentrated Assay Buffer and Dilution Buffer which are also available separately. 1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 30 minutes at 37 °C.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No. Product Price Quantity
ABG1001 A. baumannii Gyrase
100U
£105
ABG5002 A. baumannii Gyrase
500U
£386
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A. baumannii Gyrase Supercoiling Assay Kits

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Kits are available containing everything required to perform supercoiling reactions and to test inhibitors. They contain the enzyme, relaxed DNA substrate and the Assay and Dilution buffers.  

Technical Documents

Cat No. Product Price Quantity
ABS001 A.baumannii Gyrase Supercoiling Kit 1
100 U of A.baumannii gyrase and 50µg of relaxed DNA
£184
ABS002 A.baumannii Gyrase Supercoiling Kit 2
500 U of A.baumannii gyrase and 250µg of relaxed DNA
£689
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