Specific Gyrase Protein Domains

Subunits are supplied at a concentration of 1 mg/ml, purified to > 95% as assessed by SDS-PAGE. We have an extensive collection of mutants not listed here so please contact us for a full list. Different quantities and concentrations can be supplied on request.

Click on the product headings below to view details and order online.

Gyrase Subunits

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For studies involving mutant drug-resistant E. coli gyrase subunits the wild-type subunits are available separately in the following amounts. The subunits are supplied at a concentration of 1 mg/ml, purified to > 95% as assessed by SDS-PAGE. Other quantities and concentrations are available as are the individual subunits from S. aureus and M. tuberculosis gyrases; please enquire for further information.

gyrase subunit 1

Technical Documents

Cat No. Product Price Quantity
SUBA001 E. coli Gyrase A
0.1 mg
£607
SUBA005 E. coli Gyrase A
0.5 mg
£1,885
SUBA100 E. coli Gyrase A
1.0 mg
£3,358
SUBB001 E. coli Gyrase B
0.1 mg
£607
SUBB005 E. coli Gyrase B
0.5 mg
£1,885
SUBB100 E. coli Gyrase B
1.0 mg
£3,358
Custom Quote

Purified Gyrase Domains

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For structural studies the following E. coli gyrase domains are available: GyrB43, GyrB24 and GyrA59. Domains supplied at a concentration of 1 mg/ml purified to > 95% as assessed by SDS-PAGE. (see gel below for typical levels of purity)

Larger quantities/different concentrations are available, please enquire.

gyrase subunit 2

Technical Documents

Cat No. Product Price Quantity
A59001 Gyrase A59
0.1 mg
£607
A59005 Gyrase A59
0.5 mg
£1,885
A59100 Gyrase A59
1.0 mg
£3,358
B43001 Gyrase B43
0.1 mg
£607
B43005 Gyrase B43
0.5 mg
£1,885
B43100 Gyrase B43
1.0 mg
£3,358
B24001 Gyrase B24
0.1 mg
£607
B24005 Gyrase B24
0.5 mg
£1,885
B24100 Gyrase B24
1.0 mg
£3,358
Custom Quote

Drug-Resistant Topoisomerases

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We offer various quinolone- and coumarin-resistant topoisomerase mutants includingE.coliS.aureus and other organisms. The proteins can be supplied as separate subunits or as reconstituted enzyme. Typically the quinolone-resistant mutants confer 50-100 X more resistance to fluoroquinolones than the wild-type. We have more than 25 mutants in different topoisomerases and can make other mutants as requested. 

Technical Documents

Cat No. Product Price Quantity
AS83W001 E.coli GyrASer83Trp
Please enquire
POA
AS83L001 E.coli GyrASer83Leu
Please enquire
POA
BD426N001 E.coli GyrBAsp426Asn
Please enquire
POA
SAAS84L01 S.aureus GyrASer84Leu
Please enquire
POA
Custom Quote