T4 RNA Ligase 1

T4 RNA Ligase 1 is an ATP-dependent ligase that catalyses the joining of single-stranded nucleic acids. It ligates a 5'-phosphate-terminated donor (RNA or DNA) to a 3'-hydroxyl-terminated acceptor (RNA or DNA) by forming a 3'-5' phosphodiester bond. Its natural role in vivo includes the repair of tRNA molecules damaged by host antiviral responses.

Applications:

• Construction of RNA Libraries for NGS applications 
• Synthesis of Circular RNA
• Labelling or modification of 3'-end of RNA
• Enzymatic synthesis of therapeutic RNA

Bulk quantities of T4 RNA ligase are available with animal-free origin and high regulatory specifications. Please contact us for more information: [email protected] or on our Contact Us page.

One unit is defined as the amount of enzyme required to ligate 50% of an equimolar mix (125nM) of a single-stranded 5′ FAM-labeled 17-mer RNA to a 5′ phosphorylated 18-mer RNA in 20 µL at 37°C for 30 minutes.