T4 RNA Ligase 1

T4 RNA Ligase 1 catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of single-stranded RNA or DNA. It requires ATP as a cofactor.

One unit is defined as the amount of enzyme required to ligate 50% of an equimolar mix (125nM) of a single-stranded 5′ FAM-labeled 17-mer RNA to a 5′ phosphorylated 18-mer RNA in 20 µL at 37°C for 30 minutes.

Technical Documents

Cat No. Product Price Quantity
TRL101 Kit 1
2,000U T4 RNA ligase 1 (20U/uL; 100uL), 10X buffer
£75
TRL102 Kit 2
10,000U T4 RNA ligase 1 (20U/uL; 500uL), 10X buffer
£300
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