BisCat DNA

Our NEW BisCat DNA decatenation substrate consists of two supercoiled, singly-linked minicircles of 2.3 and 2.6 kb. Type II topoisomerases can decatenate the substrate to produce two supercoiled minicircle products.

Supercoiled BisCat substrate Supercoiled 2.3 + 2.6 kb minicircles Relaxed minicircles

BisCat Decatenation Assay Titration

BisCat DNA is produced using a propriety in-house method and is supplied in TE Storage Buffer at a concentration of 200 ng/µL.

Shipped on dry ice or frozen gel packs. Store BisCat DNA at -20 °C. Stable for at least 12 months.

Cat no.	Product	Price
B2001	BisCat DNA (20 µg) 20 µg of BisCat DNA	£242.00
B1002	BisCat DNA (100 µg) 100 µg of BisCat DNA	£877.00
B2003	BisCat DNA (200 µg) 200 µg of BisCat DNA	£1,239.00
B4004	BisCat DNA (400 µg) 400 µg of BisCat DNA	£1,779.00

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References

Waraich NF, Jain S, Colloms SD, Stark WM, Burton NP, Maxwell A. A novel decatenation assay for DNA topoisomerases using a singly-linked catenated substrate. Biotechniques. 2020 Nov;69(5):356-362.