All our catenated DNA substrates are available separately and have been prepared to a high standard for accurate quantification of results. They are guaranteed for use with all our topoisomerase decatenation assay kits, buffers and enzymes.
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Kinetoplast catenated DNA (kDNA) is purified from Crithidia fasciculata using the method described in Shapiro (Shapiro et al., 1999)4 and is supplied at a concentration of 100 ng/µl in 10 mM Tris-HCl (pH8.0), 1 mM EDTA. kDNA is an excellent substrate for use in topo II or topo IV decatenation assays and results can be obtained more quickly than those from relaxation assays because the decatenated mini circles can easily be resolved from the non-decatenated material which remains in the wells of the gel. Store at 4°C.