M. tuberculosis Gyrase (HIS) Decatenation Assay Kits

These kits contain all the components necessary to perform decatenation reactions. Including the enzyme, the catenated BisCat DNA or kDNA substrate, a decatenated kDNA marker (for kDNA kits), the Assay and Dilution buffers as well as 10X GSTEB - our new stop and loading buffer.

Our NEW BisCat DNA decatenation substrate consists of two supercoiled, singly-linked minicircles of 2.3 and 2.6 kb. Type II topoisomerases can decatenate the substrate to produce two supercoiled minicircle products.

Mycobacterium tuberculosis DNA gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target antibacterial drugs and can be used for screening potential novel antibacterial compounds. It is prepared by overexpressing the subunits with C-terminal polyhistidine tags in E. coli and then purified by affinity chromatography.

It is supplied as an A₂B₂ complex in Dilution Buffer. Store at -80 °C.

1 U of M. tuberculosis gyrase will decatenate 200 ng of BisCat or kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes.

Cat no.	Product	Price
MTDB001	M. tuberculosis Gyrase (HIS) BisCat Decatenation Assay Kit 1 100 U of M. tuberculosis Gyrase supplied with 5X Assay Buffer, Dilution Buffer, 20 µg BisCat DNA, and 10X GSTEB	£546.00
MTD001	M. tuberculosis Gyrase (HIS) kDNA Decatenation Assay Kit 1 100 U of M. tuberculosis Gyrase supplied with 5X Assay Buffer, Dilution Buffer, 20 µg kDNA, decatenated kDNA marker and 10X GSTEB	£524.00
MTDB002	M. tuberculosis Gyrase (HIS) BisCat Decatenation Assay Kit 2 500 U of M. tuberculosis Gyrase supplied with 5X Assay Buffer, Dilution Buffer, 100 µg BisCat, and 10X GSTEB	£2,045.00
MTD002	M. tuberculosis Gyrase (HIS) kDNA Decatenation Assay Kit 2 500 U of M. tuberculosis Gyrase supplied with 5X Assay Buffer, Dilution Buffer, 100 µg kDNA, decatenated kDNA marker and 10X GSTEB	£1,712.00

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References

Waraich NF, Jain S, Colloms SD, Stark WM, Burton NP, Maxwell A. A novel decatenation assay for DNA topoisomerases using a singly-linked catenated substrate. Biotechniques. 2020 Nov;69(5):356-362.