Anti-Human Topo IIβ Polyclonal Antibodies

Topo IIβ polyclonal primary antibodies raised against GST-tagged TOP2B C-terminal domain (CTD) in cleared rabbit serum. For HTB2B11/15, the immunogen is the full-length CTD while HTB21/25 and HT2B31/35 were raised against a truncated (last third) CTD.

The antibodies are supplied at a concentration requiring ~1:1000 dilution for Western blotting and 1:400 dilution for Immunofluorescence^1,2 (e.g. for TARDIS assays¹). The antibodies can be used for immunohistochemistry (IHC: ~1:800 dilution)².

Cat no.	Product	Price
HT2B11	Anti-TOP2B (FL-CTD) polyclonal WB & IF (4556) - 0.1 mL 0.1 mL of cleared rabbit serum containing polyclonal antibodies raised against the full-length C-terminal domain of Human Topo IIβ. These antibodies can be used of WB or IF.	£222.00
HT2B15	Anti-TOP2B (FL-CTD) polyclonal WB & IF (4556) - 0.5 mL 0.5 mL of cleared rabbit serum containing polyclonal antibodies raised against the full-length C-terminal domain of Human Topo IIβ. These antibodies can be used of WB or IF.	£823.00
HT2B21	Anti-TOP2B (TR-CTD) polyclonal for WB (4557) - 0.1 mL 0.1 mL of cleared rabbit serum containing polyclonal antibodies raised against a truncated (last third) C-terminal domain of Human Topo IIβ. Although these antibodies can be used of WB or IF, they are recommended for use in WB.	£222.00
HT2B25	Anti-TOP2B (TR-CTD) polyclonal for WB (4557) - 0.5 mL 0.5 mL of cleared rabbit serum containing polyclonal antibodies raised against a truncated (the last third) C-terminal domain of Human Topo IIβ. Although these antibodies can be used of WB or IF, they are recommended for use in WB.	£823.00
HT2B31	Anti-TOP2B (TR-CTD) polyclonal for IF (4561) - 0.1 mL 0.1 mL of cleared rabbit serum containing polyclonal antibodies raised against a truncated (last third) C-terminal domain of Human Topo IIβ. Although these antibodies can be used of WB or IF, they are recommended for use in IF.	£222.00
HT2B35	Anti-TOP2B (TR-CTD) polyclonal for IF (4561) - 0.5 mL 0.5 mL of cleared rabbit serum containing polyclonal antibodies raised against a truncated (last third) C-terminal domain of Human Topo IIβ. Although these antibodies can be used of WB or IF, they are recommended for use in IF.	£823.00

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References

Cowell IG, Austin CA. Visualization and Quantification of Topoisomerase-DNA Covalent Complexes Using the Trapped in Agarose Immunostaining (TARDIS) Assay. Methods Mol Biol. 2018;1703:301-316.
Harkin LF, Gerrelli D, Gold Diaz DC, Santos C, Alzu'bi A, Austin CA, Clowry GJ. Distinct expression patterns for type II topoisomerases IIA and IIB in the early foetal human telencephalon. J Anat. 2016 Mar;228(3):452-63.