Human Topoisomerase II
- Human Topoisomerase II
- Human Topoisomerase II Decatenation Kits
- Human Topoisomerase II Relaxation Kits
- Human Topoisomerase II Assay Kits For Cell Extracts
- High / Medium-Throughput Assay Kit - Human Topoisomerase II
Human Topoisomerase II
Human topoisomerase II is prepared by overexpressing in baculovirus-infected insect cells (Spodoptera frugiperda) and purifying it by methods developed in-house.
The enzyme is supplied at a concentration of 10 U/μl in Dilution Buffer.
Store at -80°C. (Stable for 3 months undiluted).
It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| HT201 | Human Topo II | 100 U | £55 |
| HT205 | Human Topo II | 500 U | £185 |
| HT210 | Human Topo II | 1000 U | £315 |
| HT220 | Human Topo II | 2000 U | £525 |
Human Topoisomerase II Decatenation Kits
These contain the enzyme and the kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of human topo II will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| HTK201 | Kit 1 | Contains 100 U of human topo II and 20 µg kDNA | £130 |
| HTK202 | Kit 2 | Contains 500 U of human topo II and 100 µg kDNA | £475 |
| HTK203 | Kit 3 | Contains 1000 U of human topo II and 200 µg kDNA | £765 |
| HTK204 | Kit 4 | Contains 2000 U of human topo II and 400 µg kDNA | £1210 |
Human Topoisomerase II Relaxation Kits
These contain human topo II and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of human topo II will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| HTR201 | Kit 1 | Contains 100 U of human topo II and 50 µg supercoiled DNA | £160 |
| HTR202 | Kit 2 | Contains 500 U of human topo II and 250 µg supercoiled DNA | £570 |
| HTR203 | Kit 3 | Contains 1000 U of human topo II and 500 µg supercoiled DNA | £1080 |
| HTR204 | Kit 4 | Contains 2000 U of human topo II and 1 mg supercoiled DNA | £1890 |
Human Topoisomerase II Assay Kits For Cell Extracts
These kits are designed for assaying cell extracts and partially purified fractions containing human topo II. They contain kDNA substrate, Assay buffer, Dilution buffer, decatenated and linear DNA markers and stop buffer / loading dye.
The kit components are based on the standard assay which contains 500 ng substrate DNA per assay.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| HDAK1 | Kit 1 | Contains sufficient kDNA, assay and dilution buffers for 100 assays as well as linear and decatenated marker DNA | £135 |
| HDAK2 | Kit 2 | Contains sufficient kDNA, assay and dilution buffers for 250 assays as well as linear and decatenated marker DNA | £225 |
| HDAK5 | Kit 3 | Contains sufficient kDNA, assay and dilution buffers for 500 assays as well as linear and decatenated marker DNA | £410 |
High / Medium-Throughput Assay Kit - Human Topoisomerase II
The kit is supplied with sufficient human topo II enzyme, plasmid DNA substrate, buffers and other assay components* for 100 assays. The enzyme is supplied at a concentration of 10 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use.
* Please note: The recommended fluorescence dye for this kit is SYBR Gold ® (Invitrogen) NOT SUPPLIED
More information about this assay can be found on the 'Services' page under 'High/Medium Throughput Assay'.
Kit issued with limited licence for individual use only.
Patent held by Plant Biosciences Ltd., Norwich, Norfolk, UK. ( Patent No. GB0424953.8)
| Cat No. | Product | Description | Price |
|---|---|---|---|
| TRT201 | HTS Assay Kit - Human Topo II 1-5 Kits | for 100 assays per plate | £485 |
| TRT202 | HTS Assay Kit - Human Topo II 6 Kits | for 100 assays per plate | £365 |