Purified Gyrase A and B Subunits and Domains
Gyrase Subunits
For studies involving mutant drug-resistant E. coli gyrase subunits the wild-type subunits are available separately in the following amounts. The subunits are supplied at a concentration of 1 mg/ml, purified to > 95% as assessed by SDS-PAGE. Other quantities and concentrations are available as are the individual subunits from S. aureus and M. tuberculosis gyrases; please enquire for further information.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| SUBA001 | E. coli Gyrase A | 0.1 mg | £525 |
| SUBA005 | E. coli Gyrase A | 0.5 mg | £1630 |
| SUBA100 | E. coli Gyrase A | 1.0 mg | £2900 |
| SUBB001 | E. coli Gyrase B | 0.1 mg | £525 |
| SUBB005 | E. coli Gyrase B | 0.5 mg | £1630 |
| SUBB100 | E. coli Gyrase B | 1.0 mg | £2900 |
Purified Gyrase Domains
For structural studies the following E. coli gyrase domains are available: GyrB43, GyrB24 and GyrA59. Domains supplied at a concentration of 1 mg/ml purified to > 95% as assessed by SDS-PAGE. (see gel below for typical levels of purity)
Larger quantities/different concentrations are available, please enquire.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| A59001 | Gyrase A59 | 0.1 mg | £POA |
| A59005 | Gyrase A59 | 0.5 mg | £POA |
| A59100 | Gyrase A59 | 1.0 mg | £POA |
| B43001 | Gyrase B43 | 0.1 mg | £POA |
| B43005 | Gyrase B43 | 0.5 mg | £POA |
| B43100 | Gyrase B43 | 1.0 mg | £POA |
| B24001 | Gyrase B24 | 0.1 mg | £POA |
| B24005 | Gyrase B24 | 0.5 mg | £POA |
| B24100 | Gyrase B24 | 1.0 mg | £POA |
Drug-Resistant Gyrase
We offer various quinolone- and coumarin-resistant E. coli gyrase mutants. The proteins can be supplied as separate subunits or as reconstituted enzyme. Typically the quinolone-resistant mutants confer 50-100 X more resistance to fluoroquinolones than the wild-type.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| AS83W001 | GyrASer83Trp | Please enquire | £POA |
| AS83L001 | GyrASer83Leu | Please enquire | £POA |
| BD426N001 | GyrBAsp426Asn | Please enquire | £POA |
Subunits are supplied at a concentration of 1 mg/ml, purified to > 95% as assessed by SDS-PAGE. We have an extensive collection of mutants not listed here so please contact us for a full list. Different quantities and concentrations can be supplied on request.