S. aureus Topoisomerase IV
- S. aureus Topoisomerase IV
- S. aureus Topoisomerase IV Relaxation Kits
- S. aureus Topoisomerase IV Decatenation Kits
- S. aureus Topoisomerase IV Cleavage Kits
- S. aureus Topoisomerase IV Assay Kit for Cell Extracts
- High / Medium-Throughput Assay Kit - S. aureus Topoisomerase IV
S. aureus Topoisomerase IV
Topo IV (from Staphylococcus aureus) is prepared by overexpressing the subunits in E. coli and purifying them by methods developed in-house.
The subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex at a concentration of 10 U/µl in Dilution buffer.
It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles. Store at -80ºC.
All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| SAT4001 | S. aureus Topo IV | 100 U | £110 |
| SAT4002 | S. aureus Topo IV | 500 U | £410 |
S. aureus Topoisomerase IV Relaxation Kits
These contain S. aureus topo IV and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of topo IV will relax 0.4 µg supercoiled pBR322 DNA in 30 minutes at 37°C.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| SAR4001 | Kit 1 | 100 U of S. aureus topo IV and 50 µg of Supercoiled DNA | £180 |
| SAR4002 | Kit 2 | 500 U of S. aureus topo IV and 250 µg of Supercoiled DNA | £670 |
S. aureus Topoisomerase IV Decatenation Kits
These contain S. aureus topo IV and the kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of topo IV will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| SAD4001 | Kit 1 | 100 U of S. aureus topo IV and 20 µg of kDNA | £180 |
| SAD4002 | Kit 2 | 500 U of S. aureus topo IV and 1 mg of kDNA | £670 |
S. aureus Topoisomerase IV Cleavage Kits
These kits are designed specifically for cleavage reactions. They contain S. aureus topo IV enzyme, supercoiled pBR322 DNA substrate and the Assay and Dilution buffers required for DNA cleavage reactions in addition to linearised pBR322 marker.
Cleavage specific enzyme available separately on request.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| SATC001 | Kit 1 | 100 U of S. aureus topo IV and 50 ug of supercoiled DNA | £POA |
S. aureus Topoisomerase IV Assay Kit for Cell Extracts
These kits are designed for assaying cell extracts and partially purified fractions containing over-expressed S. aureus topo IV and contain supercoiled DNA substrate, Assay buffer, Dilution buffer, control relaxed DNA and stop buffer/loading dye.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| SATE001 | Kit 1 | for 100 assays | £110 |
| SATE002 | Kit 2 | for 500 assays | £350 |
High / Medium-Throughput Assay Kit - S. aureus Topoisomerase IV
The kit is supplied with sufficient S. aureus topo IV enzyme, plasmid DNA substrate, buffers and other assay components* for 100 assays. The enzyme is supplied at a concentration of 10 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use.
* Please note: The recommended fluorescence dye for this kit is SYBR Gold ® (Invitrogen) NOT SUPPLIED
More information about this assay can be found on the 'Services' page under 'High/Medium Throughput Assay'.
Kit issued with limited licence for individual use only.
Patent held by Plant Biosciences Ltd., Norwich, Norfolk, UK. ( Patent No. GB0424953.8)
| Cat No. | Product | Description | Price |
|---|---|---|---|
| SATRIV01 | HTS Assay Kit S. aureus Topo IV 1 - 5 Kits | for 100 assays per plate | £365 |
| SATRIV02 | HTS Assay Kit S. aureus Topo IV 6+ Kits | for 100 assays per plate | £285 |