P. aeruginosa Topoisomerase IV
P. aeruginosa Topo IV
Pseudomonas aeruginosa topo IV is prepared by overexpressing the subunits in E. coli and purifying them by methods developed in-house.
The subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex at a concentration of 5 U/µl in Dilution buffer.
It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles. Store at -80ºC.
All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| PAT4001 | P. aeruginosa topo IV | 100 U | £110 |
| PAT4002 | P. aeruginosa topo IV | 500 U | £410 |
P. aeruginosa Topo IV Decatenation Assay Kits
These contain the enzyme and the kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of topo IV will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| PAD4001 | P. aeruginosa Topo IV Decatenation Kit 1 | 100 U of P. aeruginosa topo IV enzyme and 20 µg kDNA | £180 |
| PAD4002 | P. aeruginosa Topo IV Decatenation Kit 2 | 500 U of P. aeruginosa Topo IV and 100 µg kDNA | £670 |