P. aeruginosa Gyrase
P. aeruginosa Gyrase
Pseudomonas aeruginosa gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. It is prepared by overexpressing the subunits in E. coli and then purified.
It is supplied as an A2B2 complex at a concentration of 10 U/µl in Dilution Buffer. Store at -80 °C.
All enzyme is supplied with 5X concentrated Assay Buffer and Dilution Buffer which are also available separately. 1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 30 minutes at 37 °C.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| PAG1001 | P. aeruginosa Gyrase | 100 U | £90 |
| PAG5002 | P. aeruginosa Gyrase | 500 U | £330 |
P. aeruginosa Gyrase Supercoiling Assay Kits
Kits are available containing everything required to perform supercoiling reactions and to test inhibitors. They contain the enzyme, relaxed DNA substrate and the Assay and Dilution buffers.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| PAS001 | Kit 1 | 100 U of P. aeruginosa gyrase and 50 µg of relaxed DNA | £155 |
| PAS002 | Kit 2 | 500 U P. aeruginosa gyrase and 250 µg relaxed DNA | £595 |