Human Topoisomerase I
- Human Topoisomerase I
- Human Topoisomerase I Relaxation Kits
- Human Topoisomerase I Assay Kits for Cell Extracts
- High / Medium-Throughput Assay Kit - Human Topoisomerase I
Human Topoisomerase I
Human Topoisomerase I is prepared by overexpressing the enzyme in baculovirus-infected insect cells (Spodoptera frugiperda) and purifying it by methods adapted from Stewart et al., 1996. The enzyme is supplied at a concentration of 10 U/μl in Dilution Buffer.
Store at -80°C. (Stable for 3 months undiluted). It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| HT101 | Human Topo I | 100 U | £55 |
| HT105 | Human Topo I | 500 U | £185 |
| HT110 | Human Topo I | 1000 U | £315 |
| HT120 | Human Topo I | 2000 U | £525 |
Human Topoisomerase I Relaxation Kits
These contain the enzyme and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of human topo I will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| HTR101 | Kit 1 | 100 U of human topo I and 50 µg of supercoiled DNA | £135 |
| HTR102 | Kit 2 | 500 U of human topo I and 250 µg of supercoiled DNA | £475 |
| HTR103 | Kit 3 | 1000 U of human topo I and 500 µg of supercoiled DNA | £895 |
| HTR104 | Kit 4 | 2000 U of human topo I and 1 mg of supercoiled DNA | £1585 |
Human Topoisomerase I Assay Kits for Cell Extracts
These kits are designed for assaying cell extracts and partially purified fractions containing human topo I. They contain supercoiled DNA substrate, Assay buffer, Dilution buffer, relaxed DNA markers and stop buffer/loading dye.
The kit components are based on the standard human topo I assay whch contains 500 ng substrate DNA per assay.
| Cat No. | Product | Description | Price |
|---|---|---|---|
| TRAK1 | Kit 1 | Contains sufficient assay buffers, supercoiled and relaxed marker DNA for 100 assays | £135 |
| TRAK2 | Kit 2 | Contains sufficient assay buffers, supercoiled and relaxed marker DNA for 250 assays | £195 |
| TRAK3 | Kit 3 | Contains sufficient assay buffers, supercoiled and relaxed marker DNA for 500 assays | £370 |
High / Medium-Throughput Assay Kit - Human Topoisomerase I
The kit is supplied with sufficient human topo I enzyme, plasmid DNA substrate, buffers and other assay components* for 100 assays. The enzyme is supplied at a concentration of 10 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use.
* Please note: The recommended fluorescence dye for this kit is SYBR Gold ® (Invitrogen) NOT SUPPLIED
More information about this assay can be found on the 'Services' page under 'High/Medium Throughput Assay'.
Kit issued with limited licence for individual use only.
Patent held by Plant Biosciences Ltd., Norwich, Norfolk, UK. ( Patent No. GB0424953.8)
| Cat No. | Product | Description | Price |
|---|---|---|---|
| TRT101 | HTS Assay Kit - Human Topo I 1-5 kits | for 100 assays per plate | £365 |
| TRT102 | HTS Assay Kit - Human Topo I 6+ Kits | for 100 assays per plate | £270 |
References
Stewart, L., Ireton, G.C., Parker, L.H., Madden, K.R. and Champoux, J.J. (1996). Biochemical and biophysical analyses of recombinant forms of human topoisomerase I. J. Biol. Chem. 271: 7593-7601
Maxwell, A., Burton, N.P. and O’Hagan, N. (2006) High-throughput assays for DNA gyrase and other topoisomerases. Nucleic Acid Res. 34(15), e104