Monoclonal Antibodies

All antibodies are purified on a Protein A column (Ey et al., 1978)1 using the low-salt method.

The antibodies are supplied at a concentration requiring ~1:1000 dilution for Western blotting. All antibodies are stored in Phosphate Buffered Saline (PBS) pH 7.2.  Store at -20°C. (stable for at least 12 months undiluted).

7F11 Gyr A

This is an IgG2b monoclonal antibody raised against the N-terminal domain of E. coli Gyr A produced from tissue culture supernatant derived from mouse hybridoma cells.

Cat No.ProductDescriptionPrice
7F0017F11 Monoclonal0.1ml£95
7F0057F11 Monoclonal0.5ml£360
7F1007F11 Monoclonal2.0ml£1350

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4D3 Gyr A

This is an IgG2b monoclonal antibody raised against the C-terminal domain of E. coli Gyr A produced from tissue culture supernatant derived from mouse hybridoma cells.

Cat No.ProductDescriptionPrice
4D0014D3 Monoclonal0.1ml£95
4D0054D3 Monoclonal0.5ml£360
4D1004D3 Monoclonal2.0ml£1350

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9G8 Gyr B

This is an IgG2a monoclonal antibody raised against the C-terminal domain of E. coli Gyr B produced from tissue culture supernatant derived from mouse hybridoma cells.

Cat No.ProductDescriptionPrice
9G0019G8 Monoclonal0.1 ml£95
9G0059G8 Monoclonal0.5 ml£360
9G1009G8 Monoclonal2.0 ml£1350

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7D3 Gyr B

This is an IgG2a monoclonal antibody raised against the N-terminal domain of E. coli Gyr B produced from tissue culture supernatant derived from mouse hybridoma cells.

Cat No.ProductDescriptionPrice
7D0017D3 Monoclonal0.1 ml£95
7D0057D3 Monoclonal0.5 ml£360
7D1007D3 Monoclonal2.0 ml£1350

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References

  1. Ey, P.L., S.J., & Jenkin, C.R. (1978). Isolation of pure IgG1, IgG2a, and IgG2b immunoglobulins from mouse serum using Protein A sepharose. Immunochemistry. 15: 429-436

  2. Harlow, E. & Lane, D (1988). Antibodies: a laboratory manual. Cold Spring Harbor Laboratory press. Cold Spring Harbor